By Jingdong Zhang, Huangui Xiong M.D., Ph.D. (auth.), Huangui Xiong, Howard E. Gendelman (eds.)
Current Laboratory tools in Neuroscience Research is a examine handbook for either scholars and pro researchers. It makes a speciality of commonly-used options hired in neuroscience learn, offered in an easy, step by step demeanour for laboratory use. The handbook additionally deals a “blueprint” for bench-to-bedside examine designed to facilitate multidisciplinary neuroscience targets. Sections contain assurance of neurohistological recommendations, in vitro arrangements, leukocyte isolation and alertness in neuroscience, common laboratory nucleic acid and protein detections, nanomedicine, bioimaging, neuroelectrophysiology, immunohistochemistry and autoradiography, research of gene expression, and animal models.
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Additional info for Current Laboratory Methods in Neuroscience Research
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12 Epon 812 plate embedded osmificated sections, labeled neurons can be observed after embedding (arrowheads in a and b) transferred into pure Epon 812 embedding medium and bathing 2 h in the jar. (j) Prepare a hot plate at 45–60 °C, place siliconized slide on the plate, mount Epon 812-incubated section on it, and cover sections with siliconized coverslip; get rid of all air bubbles. (k) Move all slides into an oven and top coverslip with 30–50 g weight, to conduct resin polymerization that takes generally 24 h at 45 °C and 48 h at 60 °C.
Zhang • H. edu H. E. 1007/978-1-4614-8794-4_3, © Springer Science+Business Media New York 2014 45 J. Zhang and H. Xiong 46 neuroactive substances are also presented. These protocols also summarize methods of histochemical and fluorescent illumination, as well as tissue processing, embedding, and immunostaining methods for EM studies. Two major double labeling combinations used in EM observation are delineated: TMB-ST (Tetramethyl benzidine–sodium tungsten) visualization of HRP combined with ABC (Avidin-biotin-complex) histochemical stain and ABC histochemical stain combined with silver-gold pre-embedding method.
Current Laboratory Methods in Neuroscience Research by Jingdong Zhang, Huangui Xiong M.D., Ph.D. (auth.), Huangui Xiong, Howard E. Gendelman (eds.)