By G. A. Rose (auth.), G. Alan Rose MA, MD, FRCP, FRCPath, FRSC (eds.)
"Oxalate Metabolism relating to Urinary Stone" is the 3rd monograph to seem within the "Bloomsbury Series". Edited through Alan Rose, the publication describes the present scientific and biochemical positive aspects of oxalate metabolism. Its content material and course fulfil the objectives of the sequence emphasising the robust hyperlinks among simple technology and scientific medication. London Jack Tinker 30 March 1988 Preface the 1st oxalate workshop used to be held in London in 1979 and the lawsuits released privately by way of the Wellcome beginning. at the moment the significance of urinary oxalate as a probability issue extra very important for calcium oxalate stone formation than urinary calcium have been famous. however measurements of urinary oxalate nonetheless left much to be wanted and particularly the non-enzymatic conversion of ascorbate to oxalate had no longer been rediscovered in order that many measurements should have been improper. Plasma oxalate used to be nonetheless tough or very unlikely to degree by way of any moderate, available equipment and accordingly there has been nonetheless a lot argument and hypothesis in regards to the dealing with of oxalate through the kidneys. loads of paintings has been played within the final 8 years on oxalate metabolism and it accordingly looked as if it would the organisers to be a great time to carry a moment oxalate workshop.
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Extra info for Oxalate Metabolism in Relation to Urinary Stone
Measures have been taken to suppress the interference by ascorbate in a number of methods. Thus, in the ion-chromatographic procedure boric acid can be incorporated in the eluent (Scurr et a1. 1985). In an enzymatic-rateanalysis assay (Chalmers et a1. 5, is effective in the automated immobilised oxalate-oxidase assay (Kasidas and Rose 1985a) and in the Sigma assay (Kasidas and Rose 1987). Acidic ferric chloride has been advocated (Obzansky and Richardson 1983; Kasidas and Rose 1985b; Rose 1985; Crider 1985) but its effect has not been consistent in the Sigma assay (Barlow 1987; Kasidas and Rose 1987) and it can lead to a reduced life-span of the columns in the ion-chromatographic procedure (Scurr et a1.
383: 137-141 Obzansky OM, Richardson KE (1983) Quantification of urinary oxalate with oxalate oxidase from beet stems. Clin Chern 29: 1815-1819 Robertson WG, Nordin BEC (1969) Activity products in urine. In: Hodgkinson A, Nordin BEC (eds) Renal Stone Research Symposium. Churchill, London, pp 221-232 Robertson WG, Peacock M (1980) The cause of idiopathic calcium stone disease: hypercalciuria or hyperoxaluria: Nephron 26? 105-110 Robertson WG, Rutherford A (1980) Aspects of the analysis of oxalate in urine - a review.
The presence of glycollate in urine was first demonstrated by Nordmann et al. (1954). Some of the earlier techniques for urinary glycollate used paper and silica-gel chromatography and are only semi-quantitative (Nordmann et al. 1954; Osteux and Laturaze 1956; Meites 1957). Quantitative methods for determining glycollate in urine are presented below. Chemical Techniques Chemical methods based on the formation of a coloured complex between formaldehyde, produced by the reduction of glycollate, and 1,8-dihydroxynaphthalene-3,6-disulphonic acid (chromotropic acid) (Hodgkinson and Zarembski 1961; Chernoff and Richardson 1978) have been described.
Oxalate Metabolism in Relation to Urinary Stone by G. A. Rose (auth.), G. Alan Rose MA, MD, FRCP, FRCPath, FRSC (eds.)